GC Content Calculator क्या है?
Calculate GC content for DNA or RNA sequences instantly in your browser. Get GC and AT (or AU) percentages, individual base counts, and the AT/GC ratio. Paste raw sequences or FASTA text. Useful for primer design, melting temperature estimates, and sequence QC. Free, private, and no signup.
GC Content Calculator का उपयोग कैसे करें
- Choose DNA or RNA sequence type.
- Paste your sequence (A, T/U, G, C — whitespace and FASTA headers are ignored).
- Read GC% and AT/AU% in the summary cards.
- Review per-base counts and percentages in the composition table.
- Copy the summary for lab notes or bioinformatics homework.
सामान्य उपयोग
- Checking GC% before ordering PCR primers
- Comparing base composition between gene fragments
- Quick QC on downloaded FASTA sequences
अक्सर पूछे जाने वाले प्रश्न
- What is GC content?
- GC content is the percentage of guanine (G) and cytosine (C) bases in a sequence: (G + C) / total length × 100.
- Why does GC content matter?
- Higher GC content generally increases DNA melting temperature and can affect PCR primer annealing, hybridization, and genome composition studies.
- How is AT content calculated for RNA?
- For RNA, AU content uses adenine (A) and uracil (U) instead of thymine (T).
- Does it support FASTA format?
- Yes. Lines starting with > are treated as headers and removed before analysis.
- Is my sequence uploaded?
- No. All calculations run locally in your browser.
